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cardiomyocyte plating medium  (FUJIFILM)

 
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    FUJIFILM cardiomyocyte plating medium
    α- and β-myosin II can exhibit unique distributions within cardiac sarcomeres (A) Representative example of IF of endogenous β-myosin II (top) and expression of mEGFP- MYH6 (middle) in a hiCM; bottom shows overlay. Scale bars: left 10 μm, right 2 μm. (B) Line scan from yellow boxes in (A). (C) Average of all line scans (20 cells, 772 sarcomeres, 3 independent experiments). Lines show population-averaged mean value while shaded bands show ± standard error of the mean. (D) Top: expression of mNeon- MYH7 and IF of endogenous α-myosin II in a representative hiCM. Bottom: Average of all line scans (15 cells, 456 sarcomeres, 3 independent experiments). Lines show population-averaged mean value while shaded bands show ± standard error of the mean. White brackets denote individual filament stacks. Scale bar: 2 μm. (E) Distance each myosin II extended from filament center in hiCMs (β-myosin II IF: 14 cells, 280 measurements; α-myosin II IF: 15 cells, 300 measurements; mNeon- MYH7 (β expressed): 15 cells, 300 measurements; mEGFP- MYH6 (α expressed): 15 cells, 300 measurements). p -values in grey (Welch's t-test). (F) Simplified model of the potential arrangement of β-myosin II (magenta) and α-myosin II (green) in thick filaments of iPSC-derived <t>cardiac</t> <t>myocyte</t> sarcomeres. (G) Left: IF using antibodies against β-myosin II (top) and α-myosin II (bottom) in different sections of human ventricle; right: distance the fluorescent signal of each myosin extended from the filament center (β-myosin II: 280 measurements; α-myosin II: 321 measurements). Scale bar: 2 μm. p -values in grey (Welch's t-test). (H) Left: individual β-myosin II (ventricle) and α-myosin II (atrium) whole-mount immunofluorescence stains in 72 h post-fertilization zebrafish embryos; right: distance the fluorescent signal of each myosin extended from the filament center (β-myosin II: 14 embryos, 222 measurements; α-myosin II: 13 embryos, 102 measurements). White brackets in each image denote individual filament stacks. Scale bar: 2 μm. p -values in grey (Welch's t-test); (I) AlphaFold3-predicted model of α-myosin II/β-myosin II heavy chain ( MYH6 / MYH7 genes, respectively) motors + motor/lever arms, modeled with MYL2 / MYL3 (gray), which encode the ventricular essential/regulatory myosin light chains (a similar AlphaFold3-predicted model, but modeled with atrial essential/regulatory myosin light chains instead of ventricular light chains, is shown in ). N1, 2, and 3 in (E), (G), and (H) represent individual biological replicates, color-coded by replicate.
    Cardiomyocyte Plating Medium, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cardiomyocyte+plating+medium/cardiomyocyte+plating+and+maintenance+media/pmc11994913-116-1-12
    Average 90 stars, based on 1 article reviews
    cardiomyocyte plating medium - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "α- and β-myosin II can be non-uniformly distributed within the cardiac sarcomere"

    Article Title: α- and β-myosin II can be non-uniformly distributed within the cardiac sarcomere

    Journal: iScience

    doi: 10.1016/j.isci.2025.112233

    α- and β-myosin II can exhibit unique distributions within cardiac sarcomeres (A) Representative example of IF of endogenous β-myosin II (top) and expression of mEGFP- MYH6 (middle) in a hiCM; bottom shows overlay. Scale bars: left 10 μm, right 2 μm. (B) Line scan from yellow boxes in (A). (C) Average of all line scans (20 cells, 772 sarcomeres, 3 independent experiments). Lines show population-averaged mean value while shaded bands show ± standard error of the mean. (D) Top: expression of mNeon- MYH7 and IF of endogenous α-myosin II in a representative hiCM. Bottom: Average of all line scans (15 cells, 456 sarcomeres, 3 independent experiments). Lines show population-averaged mean value while shaded bands show ± standard error of the mean. White brackets denote individual filament stacks. Scale bar: 2 μm. (E) Distance each myosin II extended from filament center in hiCMs (β-myosin II IF: 14 cells, 280 measurements; α-myosin II IF: 15 cells, 300 measurements; mNeon- MYH7 (β expressed): 15 cells, 300 measurements; mEGFP- MYH6 (α expressed): 15 cells, 300 measurements). p -values in grey (Welch's t-test). (F) Simplified model of the potential arrangement of β-myosin II (magenta) and α-myosin II (green) in thick filaments of iPSC-derived cardiac myocyte sarcomeres. (G) Left: IF using antibodies against β-myosin II (top) and α-myosin II (bottom) in different sections of human ventricle; right: distance the fluorescent signal of each myosin extended from the filament center (β-myosin II: 280 measurements; α-myosin II: 321 measurements). Scale bar: 2 μm. p -values in grey (Welch's t-test). (H) Left: individual β-myosin II (ventricle) and α-myosin II (atrium) whole-mount immunofluorescence stains in 72 h post-fertilization zebrafish embryos; right: distance the fluorescent signal of each myosin extended from the filament center (β-myosin II: 14 embryos, 222 measurements; α-myosin II: 13 embryos, 102 measurements). White brackets in each image denote individual filament stacks. Scale bar: 2 μm. p -values in grey (Welch's t-test); (I) AlphaFold3-predicted model of α-myosin II/β-myosin II heavy chain ( MYH6 / MYH7 genes, respectively) motors + motor/lever arms, modeled with MYL2 / MYL3 (gray), which encode the ventricular essential/regulatory myosin light chains (a similar AlphaFold3-predicted model, but modeled with atrial essential/regulatory myosin light chains instead of ventricular light chains, is shown in ). N1, 2, and 3 in (E), (G), and (H) represent individual biological replicates, color-coded by replicate.
    Figure Legend Snippet: α- and β-myosin II can exhibit unique distributions within cardiac sarcomeres (A) Representative example of IF of endogenous β-myosin II (top) and expression of mEGFP- MYH6 (middle) in a hiCM; bottom shows overlay. Scale bars: left 10 μm, right 2 μm. (B) Line scan from yellow boxes in (A). (C) Average of all line scans (20 cells, 772 sarcomeres, 3 independent experiments). Lines show population-averaged mean value while shaded bands show ± standard error of the mean. (D) Top: expression of mNeon- MYH7 and IF of endogenous α-myosin II in a representative hiCM. Bottom: Average of all line scans (15 cells, 456 sarcomeres, 3 independent experiments). Lines show population-averaged mean value while shaded bands show ± standard error of the mean. White brackets denote individual filament stacks. Scale bar: 2 μm. (E) Distance each myosin II extended from filament center in hiCMs (β-myosin II IF: 14 cells, 280 measurements; α-myosin II IF: 15 cells, 300 measurements; mNeon- MYH7 (β expressed): 15 cells, 300 measurements; mEGFP- MYH6 (α expressed): 15 cells, 300 measurements). p -values in grey (Welch's t-test). (F) Simplified model of the potential arrangement of β-myosin II (magenta) and α-myosin II (green) in thick filaments of iPSC-derived cardiac myocyte sarcomeres. (G) Left: IF using antibodies against β-myosin II (top) and α-myosin II (bottom) in different sections of human ventricle; right: distance the fluorescent signal of each myosin extended from the filament center (β-myosin II: 280 measurements; α-myosin II: 321 measurements). Scale bar: 2 μm. p -values in grey (Welch's t-test). (H) Left: individual β-myosin II (ventricle) and α-myosin II (atrium) whole-mount immunofluorescence stains in 72 h post-fertilization zebrafish embryos; right: distance the fluorescent signal of each myosin extended from the filament center (β-myosin II: 14 embryos, 222 measurements; α-myosin II: 13 embryos, 102 measurements). White brackets in each image denote individual filament stacks. Scale bar: 2 μm. p -values in grey (Welch's t-test); (I) AlphaFold3-predicted model of α-myosin II/β-myosin II heavy chain ( MYH6 / MYH7 genes, respectively) motors + motor/lever arms, modeled with MYL2 / MYL3 (gray), which encode the ventricular essential/regulatory myosin light chains (a similar AlphaFold3-predicted model, but modeled with atrial essential/regulatory myosin light chains instead of ventricular light chains, is shown in ). N1, 2, and 3 in (E), (G), and (H) represent individual biological replicates, color-coded by replicate.

    Techniques Used: Expressing, Derivative Assay, Immunofluorescence

    Related Articles

    Expressing:

    Article Title: α- and β-myosin II can be non-uniformly distributed within the cardiac sarcomere
    Article Snippet: at a density of 50,000 cells per well in 96-well polystyrene cell-culture plates pre-coated for 2 h with gelatin (EMD Millipore; catalog # ES-006-B). .. The Cardiomyocyte Plating Medium was replaced with pre-warmed iCell Cardiomyocytes Maintenance Medium (Fujifilm Cellular Dynamics; catalog #M1003) 5 h post-thawing, then again every 48 h. The cells were kept at 37°C with 5% CO2. .. All hiCMs used in this study were generated from fibroblast cells isolated from a healthy female from European descent.

    Article Title: α- and β-myosin II can be non-uniformly distributed within the cardiac sarcomere
    Article Snippet: obtained from Fujifilm Cellular Dynamics (iCell Cardiomyocytes2; catalog # CMM-100-012-000.5) and cultured following the manufacturer’s guidelines. .. In brief, the hiCMs were thawed and plated in 100uL per well Cardiomyocyte Plating Medium (Fujifilm Cellular Dynamics; catalog #R1151) at a density of 50,000 cells per well in 96-well polystyrene cell-culture plates pre-coated for 2 h with gelatin (EMD Millipore; catalog # ES-006-B). .. The Cardiomyocyte Plating Medium was replaced with pre-warmed iCell Cardiomyocytes Maintenance Medium (Fujifilm Cellular Dynamics; catalog #M1003) 5 h

    Derivative Assay:

    Article Title: α- and β-myosin II can be non-uniformly distributed within the cardiac sarcomere
    Article Snippet: at a density of 50,000 cells per well in 96-well polystyrene cell-culture plates pre-coated for 2 h with gelatin (EMD Millipore; catalog # ES-006-B). .. The Cardiomyocyte Plating Medium was replaced with pre-warmed iCell Cardiomyocytes Maintenance Medium (Fujifilm Cellular Dynamics; catalog #M1003) 5 h post-thawing, then again every 48 h. The cells were kept at 37°C with 5% CO2. .. All hiCMs used in this study were generated from fibroblast cells isolated from a healthy female from European descent.

    Article Title: α- and β-myosin II can be non-uniformly distributed within the cardiac sarcomere
    Article Snippet: obtained from Fujifilm Cellular Dynamics (iCell Cardiomyocytes2; catalog # CMM-100-012-000.5) and cultured following the manufacturer’s guidelines. .. In brief, the hiCMs were thawed and plated in 100uL per well Cardiomyocyte Plating Medium (Fujifilm Cellular Dynamics; catalog #R1151) at a density of 50,000 cells per well in 96-well polystyrene cell-culture plates pre-coated for 2 h with gelatin (EMD Millipore; catalog # ES-006-B). .. The Cardiomyocyte Plating Medium was replaced with pre-warmed iCell Cardiomyocytes Maintenance Medium (Fujifilm Cellular Dynamics; catalog #M1003) 5 h

    Immunofluorescence:

    Article Title: α- and β-myosin II can be non-uniformly distributed within the cardiac sarcomere
    Article Snippet: at a density of 50,000 cells per well in 96-well polystyrene cell-culture plates pre-coated for 2 h with gelatin (EMD Millipore; catalog # ES-006-B). .. The Cardiomyocyte Plating Medium was replaced with pre-warmed iCell Cardiomyocytes Maintenance Medium (Fujifilm Cellular Dynamics; catalog #M1003) 5 h post-thawing, then again every 48 h. The cells were kept at 37°C with 5% CO2. .. All hiCMs used in this study were generated from fibroblast cells isolated from a healthy female from European descent.

    Article Title: α- and β-myosin II can be non-uniformly distributed within the cardiac sarcomere
    Article Snippet: obtained from Fujifilm Cellular Dynamics (iCell Cardiomyocytes2; catalog # CMM-100-012-000.5) and cultured following the manufacturer’s guidelines. .. In brief, the hiCMs were thawed and plated in 100uL per well Cardiomyocyte Plating Medium (Fujifilm Cellular Dynamics; catalog #R1151) at a density of 50,000 cells per well in 96-well polystyrene cell-culture plates pre-coated for 2 h with gelatin (EMD Millipore; catalog # ES-006-B). .. The Cardiomyocyte Plating Medium was replaced with pre-warmed iCell Cardiomyocytes Maintenance Medium (Fujifilm Cellular Dynamics; catalog #M1003) 5 h



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    FUJIFILM cardiomyocyte plating medium
    α- and β-myosin II can exhibit unique distributions within cardiac sarcomeres (A) Representative example of IF of endogenous β-myosin II (top) and expression of mEGFP- MYH6 (middle) in a hiCM; bottom shows overlay. Scale bars: left 10 μm, right 2 μm. (B) Line scan from yellow boxes in (A). (C) Average of all line scans (20 cells, 772 sarcomeres, 3 independent experiments). Lines show population-averaged mean value while shaded bands show ± standard error of the mean. (D) Top: expression of mNeon- MYH7 and IF of endogenous α-myosin II in a representative hiCM. Bottom: Average of all line scans (15 cells, 456 sarcomeres, 3 independent experiments). Lines show population-averaged mean value while shaded bands show ± standard error of the mean. White brackets denote individual filament stacks. Scale bar: 2 μm. (E) Distance each myosin II extended from filament center in hiCMs (β-myosin II IF: 14 cells, 280 measurements; α-myosin II IF: 15 cells, 300 measurements; mNeon- MYH7 (β expressed): 15 cells, 300 measurements; mEGFP- MYH6 (α expressed): 15 cells, 300 measurements). p -values in grey (Welch's t-test). (F) Simplified model of the potential arrangement of β-myosin II (magenta) and α-myosin II (green) in thick filaments of iPSC-derived <t>cardiac</t> <t>myocyte</t> sarcomeres. (G) Left: IF using antibodies against β-myosin II (top) and α-myosin II (bottom) in different sections of human ventricle; right: distance the fluorescent signal of each myosin extended from the filament center (β-myosin II: 280 measurements; α-myosin II: 321 measurements). Scale bar: 2 μm. p -values in grey (Welch's t-test). (H) Left: individual β-myosin II (ventricle) and α-myosin II (atrium) whole-mount immunofluorescence stains in 72 h post-fertilization zebrafish embryos; right: distance the fluorescent signal of each myosin extended from the filament center (β-myosin II: 14 embryos, 222 measurements; α-myosin II: 13 embryos, 102 measurements). White brackets in each image denote individual filament stacks. Scale bar: 2 μm. p -values in grey (Welch's t-test); (I) AlphaFold3-predicted model of α-myosin II/β-myosin II heavy chain ( MYH6 / MYH7 genes, respectively) motors + motor/lever arms, modeled with MYL2 / MYL3 (gray), which encode the ventricular essential/regulatory myosin light chains (a similar AlphaFold3-predicted model, but modeled with atrial essential/regulatory myosin light chains instead of ventricular light chains, is shown in ). N1, 2, and 3 in (E), (G), and (H) represent individual biological replicates, color-coded by replicate.
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    α- and β-myosin II can exhibit unique distributions within cardiac sarcomeres (A) Representative example of IF of endogenous β-myosin II (top) and expression of mEGFP- MYH6 (middle) in a hiCM; bottom shows overlay. Scale bars: left 10 μm, right 2 μm. (B) Line scan from yellow boxes in (A). (C) Average of all line scans (20 cells, 772 sarcomeres, 3 independent experiments). Lines show population-averaged mean value while shaded bands show ± standard error of the mean. (D) Top: expression of mNeon- MYH7 and IF of endogenous α-myosin II in a representative hiCM. Bottom: Average of all line scans (15 cells, 456 sarcomeres, 3 independent experiments). Lines show population-averaged mean value while shaded bands show ± standard error of the mean. White brackets denote individual filament stacks. Scale bar: 2 μm. (E) Distance each myosin II extended from filament center in hiCMs (β-myosin II IF: 14 cells, 280 measurements; α-myosin II IF: 15 cells, 300 measurements; mNeon- MYH7 (β expressed): 15 cells, 300 measurements; mEGFP- MYH6 (α expressed): 15 cells, 300 measurements). p -values in grey (Welch's t-test). (F) Simplified model of the potential arrangement of β-myosin II (magenta) and α-myosin II (green) in thick filaments of iPSC-derived <t>cardiac</t> <t>myocyte</t> sarcomeres. (G) Left: IF using antibodies against β-myosin II (top) and α-myosin II (bottom) in different sections of human ventricle; right: distance the fluorescent signal of each myosin extended from the filament center (β-myosin II: 280 measurements; α-myosin II: 321 measurements). Scale bar: 2 μm. p -values in grey (Welch's t-test). (H) Left: individual β-myosin II (ventricle) and α-myosin II (atrium) whole-mount immunofluorescence stains in 72 h post-fertilization zebrafish embryos; right: distance the fluorescent signal of each myosin extended from the filament center (β-myosin II: 14 embryos, 222 measurements; α-myosin II: 13 embryos, 102 measurements). White brackets in each image denote individual filament stacks. Scale bar: 2 μm. p -values in grey (Welch's t-test); (I) AlphaFold3-predicted model of α-myosin II/β-myosin II heavy chain ( MYH6 / MYH7 genes, respectively) motors + motor/lever arms, modeled with MYL2 / MYL3 (gray), which encode the ventricular essential/regulatory myosin light chains (a similar AlphaFold3-predicted model, but modeled with atrial essential/regulatory myosin light chains instead of ventricular light chains, is shown in ). N1, 2, and 3 in (E), (G), and (H) represent individual biological replicates, color-coded by replicate.
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    Image Search Results


    α- and β-myosin II can exhibit unique distributions within cardiac sarcomeres (A) Representative example of IF of endogenous β-myosin II (top) and expression of mEGFP- MYH6 (middle) in a hiCM; bottom shows overlay. Scale bars: left 10 μm, right 2 μm. (B) Line scan from yellow boxes in (A). (C) Average of all line scans (20 cells, 772 sarcomeres, 3 independent experiments). Lines show population-averaged mean value while shaded bands show ± standard error of the mean. (D) Top: expression of mNeon- MYH7 and IF of endogenous α-myosin II in a representative hiCM. Bottom: Average of all line scans (15 cells, 456 sarcomeres, 3 independent experiments). Lines show population-averaged mean value while shaded bands show ± standard error of the mean. White brackets denote individual filament stacks. Scale bar: 2 μm. (E) Distance each myosin II extended from filament center in hiCMs (β-myosin II IF: 14 cells, 280 measurements; α-myosin II IF: 15 cells, 300 measurements; mNeon- MYH7 (β expressed): 15 cells, 300 measurements; mEGFP- MYH6 (α expressed): 15 cells, 300 measurements). p -values in grey (Welch's t-test). (F) Simplified model of the potential arrangement of β-myosin II (magenta) and α-myosin II (green) in thick filaments of iPSC-derived cardiac myocyte sarcomeres. (G) Left: IF using antibodies against β-myosin II (top) and α-myosin II (bottom) in different sections of human ventricle; right: distance the fluorescent signal of each myosin extended from the filament center (β-myosin II: 280 measurements; α-myosin II: 321 measurements). Scale bar: 2 μm. p -values in grey (Welch's t-test). (H) Left: individual β-myosin II (ventricle) and α-myosin II (atrium) whole-mount immunofluorescence stains in 72 h post-fertilization zebrafish embryos; right: distance the fluorescent signal of each myosin extended from the filament center (β-myosin II: 14 embryos, 222 measurements; α-myosin II: 13 embryos, 102 measurements). White brackets in each image denote individual filament stacks. Scale bar: 2 μm. p -values in grey (Welch's t-test); (I) AlphaFold3-predicted model of α-myosin II/β-myosin II heavy chain ( MYH6 / MYH7 genes, respectively) motors + motor/lever arms, modeled with MYL2 / MYL3 (gray), which encode the ventricular essential/regulatory myosin light chains (a similar AlphaFold3-predicted model, but modeled with atrial essential/regulatory myosin light chains instead of ventricular light chains, is shown in ). N1, 2, and 3 in (E), (G), and (H) represent individual biological replicates, color-coded by replicate.

    Journal: iScience

    Article Title: α- and β-myosin II can be non-uniformly distributed within the cardiac sarcomere

    doi: 10.1016/j.isci.2025.112233

    Figure Lengend Snippet: α- and β-myosin II can exhibit unique distributions within cardiac sarcomeres (A) Representative example of IF of endogenous β-myosin II (top) and expression of mEGFP- MYH6 (middle) in a hiCM; bottom shows overlay. Scale bars: left 10 μm, right 2 μm. (B) Line scan from yellow boxes in (A). (C) Average of all line scans (20 cells, 772 sarcomeres, 3 independent experiments). Lines show population-averaged mean value while shaded bands show ± standard error of the mean. (D) Top: expression of mNeon- MYH7 and IF of endogenous α-myosin II in a representative hiCM. Bottom: Average of all line scans (15 cells, 456 sarcomeres, 3 independent experiments). Lines show population-averaged mean value while shaded bands show ± standard error of the mean. White brackets denote individual filament stacks. Scale bar: 2 μm. (E) Distance each myosin II extended from filament center in hiCMs (β-myosin II IF: 14 cells, 280 measurements; α-myosin II IF: 15 cells, 300 measurements; mNeon- MYH7 (β expressed): 15 cells, 300 measurements; mEGFP- MYH6 (α expressed): 15 cells, 300 measurements). p -values in grey (Welch's t-test). (F) Simplified model of the potential arrangement of β-myosin II (magenta) and α-myosin II (green) in thick filaments of iPSC-derived cardiac myocyte sarcomeres. (G) Left: IF using antibodies against β-myosin II (top) and α-myosin II (bottom) in different sections of human ventricle; right: distance the fluorescent signal of each myosin extended from the filament center (β-myosin II: 280 measurements; α-myosin II: 321 measurements). Scale bar: 2 μm. p -values in grey (Welch's t-test). (H) Left: individual β-myosin II (ventricle) and α-myosin II (atrium) whole-mount immunofluorescence stains in 72 h post-fertilization zebrafish embryos; right: distance the fluorescent signal of each myosin extended from the filament center (β-myosin II: 14 embryos, 222 measurements; α-myosin II: 13 embryos, 102 measurements). White brackets in each image denote individual filament stacks. Scale bar: 2 μm. p -values in grey (Welch's t-test); (I) AlphaFold3-predicted model of α-myosin II/β-myosin II heavy chain ( MYH6 / MYH7 genes, respectively) motors + motor/lever arms, modeled with MYL2 / MYL3 (gray), which encode the ventricular essential/regulatory myosin light chains (a similar AlphaFold3-predicted model, but modeled with atrial essential/regulatory myosin light chains instead of ventricular light chains, is shown in ). N1, 2, and 3 in (E), (G), and (H) represent individual biological replicates, color-coded by replicate.

    Article Snippet: The Cardiomyocyte Plating Medium was replaced with pre-warmed iCell Cardiomyocytes Maintenance Medium (Fujifilm Cellular Dynamics; catalog #M1003) 5 h post-thawing, then again every 48 h. The cells were kept at 37°C with 5% CO2.

    Techniques: Expressing, Derivative Assay, Immunofluorescence

    Precision of ddPCR assay using the same RNA samples prepared from iPSC-spiked cardiomyocytes and human heart in step 1 study.

    Journal: Stem Cells Translational Medicine

    Article Title: Detection of residual pluripotent stem cells in cell therapy products utilizing droplet digital PCR: an international multisite evaluation study

    doi: 10.1093/stcltm/szae058

    Figure Lengend Snippet: Precision of ddPCR assay using the same RNA samples prepared from iPSC-spiked cardiomyocytes and human heart in step 1 study.

    Article Snippet: Cryopreserved iCell CM was thawed with iCell Cardiomyocytes Plating Medium (FUJIFILM Cellular Dynamics) according to the manufacturer’s instructions.

    Techniques: